IN VITRO STUDY OF THE INFLUENCE OF VARIOUS CONCENTRATIONS OF DINITROSYL IRON COMPLEXES ON THE ACTIVITY OF ANTIOXIDANT ENZYMES IN THE BLOOD

Abstract

The effect of various concentrations of dinitrosyl iron complexes (DNICs) on the activity of superoxide dismutase (SOD), catalase and glutathione reductase (GR) was studied under in vitro conditions. The experiment was carried out using preserved blood with a freshly pre-pared aqueous solution of DNICs added (concentration - 5 mmol / l) having a ratio of 1: 150, 1: 100, 1:50 and 1:25. Synthesis of DNICs was performed according to the method described by A.F. Vanina et al. (2005). A multidirectional and dose-dependent effect of DNICs on the activity of antioxidant enzymes of donor blood was revealed. Under the influence of DNICs with a ratio of 1: 150, 1: 100 and 1:50, an increase in SOD activity was observed. The pres-ence of DNICs in the donor blood at a ratio of 1:25 caused a decrease in the total and specific activity of SOD. An increase both in total and specific activities of GR with the addition of DNICs to donor blood in all presented ratios was noted. A decrease in the total and specific catalase activity with the addition of DNICs to the donor blood in a ratio of 1: 150 was observed against the background of the growth of the total activity of the enzyme under the influence of DNICs at high concentrations. An increase in the specific activity of catalase in the presence of DNICs with ratio 1: 100 and 1:50 was revealed. It has been shown that DNICs have antioxidant properties, manifesting through an increase in the activity of SOD, catalase, and GR when injected into donor blood under in vitro conditions in the ratios 1: 100 and 1:50.