IN VITRO STUDY OF THE EFFECT OF AQUEOUS POTATO EXTRACTS ON THE VITALITY AND MORPHOLOGY OF THE CAUSATIVE AGENT OF RING ROT
https://doi.org/10.21285/2227-2925-2017-7-4-79-86
Abstract
The effect of an aqueous extract of different varieties of potato plants on the morphological structure and viability of the causative agent of the ring rot - globulin-positive bacterium Clavibacter michiganensis ssp. sepedonicus (Cms) was studied. Six varieties of Solanum tuberosum L. potato plants were studied in vitro: Bellarosa, Zhukovsky ranniy, Lugovskoy, Lukyanovsky, Rosara, Sarma. All varieties differed in their resistance to pathogens. Bacteria were incubated in an aqueous extract of plants for 2 days, then the number of cells in the field of vision, the mortality rate and the width and length of bacterial cells were analysed. The obtained data indicated that the number of cells in the field of vision of a microscope following incubation in an aqueous potato extract, as well as the percentage of living cells, depends on the type of potato. The morphological characteristics (width and length of cells) of the bacterium also changed depending on the type of potato plants. The technique presented in the article is proposed as a means for quickly identifying potato resistance to pathogenic bacteria.
About the Authors
O. A. Nozhkina
Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch, Russian Academy of Sciences
Russian Federation
Russian Federation
A. I. Perfileva
Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch, Russian Academy of Sciences
Russian Federation
Russian Federation
A. V. Sidorov
Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch, Russian Academy of Sciences
Russian Federation
Russian Federation
I. A. Graskova
Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch, Russian Academy of Sciences
Russian Federation
Russian Federation
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For citations:
Nozhkina O.A., Perfileva A.I., Sidorov A.V., Graskova I.A. IN VITRO STUDY OF THE EFFECT OF AQUEOUS POTATO EXTRACTS ON THE VITALITY AND MORPHOLOGY OF THE CAUSATIVE AGENT OF RING ROT. Proceedings of Universities. Applied Chemistry and Biotechnology. 2017;7(4):79-86. (In Russ.) https://doi.org/10.21285/2227-2925-2017-7-4-79-86
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