Influence of new humic preparation on remediation of oil-contaminated soil
https://doi.org/10.21285/2227-2925-2022-12-2-310-320
Abstract
A humic preparation obtained through alkaline extraction of humic-containing raw materials at the All-Russian Research Institute of Reclaimed Lands (branch of the V. V. Dokuchaev Soil Science Institute) is presented. The aim of the work is to study the effect of the humic preparation – named BoGum – on the remediation of oil-contaminated podzols. In a model experiment for soil remediation, artificially contaminated with oil in an amount of 5%, BoGum was used in 3 doses: as a stimulant of aboriginal microflora (300 l/ha), as a fertiliser (3000 l/ha), and as a sorbent (30000 l/ha). BoGum was found to be more effective as a stimulator of aboriginal microflora, whose maximum was observed one month following application, while the degree of destruction of oil over the two months of the experiment was 40.1%. Over the course of the experiment, it was determined that humic substances of the preparation were a more affordable source of nutrition for the soil microflora when BoGum was used as a sorbent; the breakdown of oil was the lowest when petroleum hydrocarbons were inaccessible to microorganisms due to sorption. The maximum degree of breakdown (44.9%) occurred with the combined use of the microbial preparation MicroBak (development of the Institute of Biochemistry and Physiology of Microorganisms of the Russian Academy of Sciences) and the humic preparation BoGum at a dosage of 300 l/ha. The preparation demonstrated a synergistic effect, which affected the active development of both hydrocarbon-oxidising microorganisms and aboriginal microflora. The percentage of the hydrocarbon-oxidising microorganisms from the total number of heterotrophs in all experimental variants was maximum in two weeks; this was reduced at different intensities. Calculated multiple regression equations were used to estimate the physiological influence of the groups of microorganisms on the degree of degradation being investigated.
About the Authors
N. V. Fomicheva
FRC V. V. Dokuchaev Soil Science Institute
Russian Federation
Russian Federation
Natalia V. Fomicheva, Cand. Sci. (Biology), Leading Researcher
27, Emmauss Village, Kalininsky District, 170530
Yu. D. Smirnova
FRC V. V. Dokuchaev Soil Science Institute
Russian Federation
Russian Federation
Yulia D. Smirnova, Cand. Sci. (Biology), Deputy Director for Science
27, Emmauss Village, Kalininsky District, 170530
G. Yu. Rabinovich
FRC V. V. Dokuchaev Soil Science Institute
Russian Federation
Russian Federation
Galina Yu. Rabinovich, Dr. Sci. (Biology), Professor, Chief Researcher
27, Emmauss Village, Kalininsky District, 170530
References
1. Balandin D. A., Pytkin A. N., Tarasov N. M. Integrated application of biotechnologies in achieving sustainable development of rural areas of the region: monograph. Ekaterinburg: Institut ekonomiki Ural'skogo otdeleniya Rossiiskoi akademii nauk; 2014. 166 p. (In Russian).
2. Akhmadiev М. V. Using of aboriginal hydrocarbons-oxidizing microorganisms in the bioremediation of contaminated soil and ground. Vestnik Permskogo natsional'nogo issledovatel'skogo politekhnicheskogo universiteta. Prikladnaya ekologiya. Urbanistika = Bulletin of Perm National Research Polytechnic University. Applied Ecology. Urban Development. 2014;(2): 119-130. (In Russian).
3. Tsivadze A. Y., Fridman A. Y., Novikov A. K., Polyakova I. Y., Tumanyan B. P., Sudarkin A. P. Prospective preparations for accelerated bioremediation of oil-contaminated soils. Khimiya i tekhnologiya topliv i masel = Chemistry and Technology of Fuels and Oils. 2020;(4):53-56. (In Russian).
4. Cherdakova A. S., Galchenko S. V. Analysis of prospects for the use of humic drugs for restoration of oil-contaminated soils. In: Aktual'nye problemy ekologii i prirodopol'zovaniya: sbornik nauchnykh trudov XXI Mezhdunarodnoi nauchnoprakticheskoi konferentsii = Actual problems of ecology and nature management: a collection of scientific papers of the XXI International Scientific and Practical Conference. April-September 2020, Moscow. Moscow: RUDN; 2020, p. 234-240. (In Russian).
5. Kulagin A. A., Ganeev I. G., Sukhova S. V., Ziganshin Z. U. Safety technology use reclamation contaminated, violations and degraded lands using humic substances. Izvestiya Ufimskogo nauchnogo tsentra RAN = Proceedings of the RAS Ufa Scientific Centre. 2015;(4-1):83-85. (In Russian).
6. Ivanov A. A., Yudina N. V., Matis E. Y., Mal'tseva E. V., Svarovskaya L. I. Stimulation of the activity of microorganisms by humin preparations in oil-polluted soils. Pochvovedenie = Eurasian Soil Science. 2010;(2):229-234. (In Russian).
7. Miroshnichenko Y. S., Myasoedova T. N., Proshina M. A. Estimation of detoxification ability of humic substances in oil contaminated soils. Ekologiya promyshlennogo proizvodstva = Iindustrial Ecology. 2016;(2):57-61. (In Russian).
8. Vaysman Ya. I., Glushankova I. S., Rudakova L. V., Kumi V. V., Tokarev I. P. Development of technology remediation soil contaminated by oil using GUMIKOM. Neftyanoe khozyaistvo = Oil Industry. 2013;(10):128-131. (In Russian).
9. Stepanov A. A., Gosse D. D., Panina M. A. Application of humin preparation “Peter Peat” for detoxication and reconstruction of oil-polluted soil. Problemy agrokhimii i ekologii = Agrochemistry and ecology problems. 2018;(1):55-57. (In Russian).
10. Salem K. M., Perminova I. V., Grechishcheva N. Yu., Mouryguina V. P., Meshcheryakov S. V. Biorevegetation of oily soils by humic preparations. Ekologiya i promyshlennost' Rossii = Ecology and Industry of Russia. 2003;(4):19-21. (In Russian).
11. Grechishcheva N. Yu., Fekhretdinova D. R., Murygina V. P., Gaydamaka S. N. Evaluation of the effectiveness of humic substances use as washing agents of oil-contaminated soils. Zashchita okruzhayushchei sredy v neftegazovom komplekse = Environmental protection in oil and gas complex. 2019;(6):22-26. (In Russian). https://doi.org/10.33 285/2411-7013-2019-6(291)-22-26.
12. Filatov D. A., Ivanov A. A., Svarovskaya L. I., Yudina N. V. Effect of light-corrective film and humic acids on the biochemical oxidation of petroleum in the soil. Agrokhimiya = Eurasian Soil Science. 2011;(10):76-82. (In Russian).
13. Gretchischeva N. Yu., Perminova I. V., Mescheryakov S. V. Humic compounds in treatment of oil contaminated environments. Ekologiya i promyshlennost' Rossii = Ecology and Industry of Russia. 2016;20(1):30-36. (In Russian). https://doi.org/ 10.18412/1816-0395-2016-1-30-36.
14. Fomicheva N. V., Rabinovich G. Yu., Smirnova Yu. D. Influence of technological methods of application of the humic preparation on the productivity of spring wheat. Dostizheniya nauki i tekhniki APK = Achievements of Science and Technology in AgroIndustrial Complex. 2020;34(9):53-58. (In Russian). https://doi.org/10.24411/0235-2451-2020-10910.
15. Rabinovich G. Yu., Fomicheva N. V., Smirnova Yu. D. Efficiency of liquid-phase biological preparations when cultivating carrots on peat soils. Vestsі natsyyanal'nai akademіі navuk Belarusі. Seryya agrarnykh navuk = Proceedings of the National Academy of Sciences of Belarus. Agrarian Series. 2021;59(3):319- 329. (In Russian). https://doi.org/10.29235/1817-7204- 2021-59-3-319-329.
16. Fomicheva N. V., Rabinovich G. Yu. Method of producing liquid humic fertilizer. Patent RF, no. 2691693; 2019. (In Russian).
17. Karimova V. T., Dmitrieva E. D., Nechaeva I. A. The effect of humic substances from different origin peats of the tula region on the growth of microbial degraders of oil Rhodococcus Erythropolis S67 and Rhodococcus Erythropolis X5. Izvestiya Tul'skogo gosudarstvennogo universiteta. Estestvennye nauki = Proceedings of the Tula State University. Natural Sciences. 2017;(2):60-68. (In Russian).
18. Cherdakova A. S., Gal'chenko S. V., Sipataya R. A. Influence of humic preparations on the processes of bioremediation of oil-contaminated aquatic environments. In: Novye tekhnologii v uchebnom protsesse i proizvodstve: materialy XIX Mezhdunarodnoi nauchno-tekhnicheskoi konferentsii = New Technologies in the Educational Process and Production: Materials of the XIX International Scientific and Technical Conference. 14-16 April 2021, Ryazan. Ryazan; 2021, p. 221-222. (In Russian).
19. Drugov Yu. S., Rodin A. A. Analysis of contaminated soil and hazardous waste. Moscow: Binom. Laboratoriya znanii; 2007. 424 p. (In Russian).
20. Kurochkina G. N., Amelin A. A., Shkidchenko A. N. The effect of a new biopreparation on the remediation of oil-contaminated gray forest soil. Pochvovedenie = Eurasian Soil Science. 2004; (10):1241-1249. (In Russian).
21. Ovsyannikova V. S., Filatov D. A., Altunina L. K., Svarovskaya L. I. Biodestruction of hydrocarbons of highly viscous petroleum with soil microorganisms. Khimiya v interesakh ustoichivogo razvitiya = Chemistry for Sustainable Development. 2014;22(5):489- 495. (In Russian).
22. Nechaeva I. A., Filonov А. E., Akhmetov L. I., Puntus I. F., Boronin А. M. Stimulation of microbial destruction of oil by introduction of bacterial association and mineral fertilizer in soil under laboratory and field conditions. Biotekhnologiya = Biotechnology in Russia. 2009;(1):64-70. (In Russian).
23. Vetrova A. A., Zabelin V. A., Ivanova A. A., Adamenko L. A., Delegan Y. A., Petrikov K. V. Oil biodegradation by consortium of oil degrading microorganisms in laboratory model systems. Yug Rossii: ekologiya, razvitie = South of Russia: ecology, development. 2018;13(1):184-198. (In Russian). https://doi. org/10.18470/1992-1098-2018-1-184-198.
24. Rudenko E. Yu. Effects of crude oil on the biological activity of chernozem soils. Izvestiya Vuzov. Prikladnaya Khimiya i Biotekhnologiya = Proceedings of Universities. Applied Chemistry and Biotechnology. 2020;10(4):719-727. (In Russian). https://doi.org/10.21 285/2227-2925-2020-10-4-719-727.
25. Evdokimova G. A. Soil microbiota as a factor of soil resistance to contamination. Teoreticheskaya i prikladnaya ekologiya = Theoretical and Applied Ecology. 2014;(2):17-24. (In Russian).
26. Ivanov A. A., Yudina N. V., Mal'tseva E. V., Matis E. Ya. Study of biostimulating and detoxifying properties of humic acids of various origins in oilcontaminated soil. Khimija Rastitel'nogo Syr'ja. 2007;(1):99-103. (In Russian).
27. Yakimenko O. S., Terekhova V. A. Humic preparations and the assessment of their biological activity for certification purposes. Pochvovedenie = Eurasian Soil Science. 2011;(11):1334-1343. (In Russian).
28. Tikhonov V. V., Yakushev A. V., Zavgorodnyaya Y. A., Byzov B. A., Demin V. V. Effects of humic acids on the growth of bacteria. Pochvovedenie = Eurasian Soil Science. 2010;(3):333-341. (In Russian).
29. Pleshakova E. V. Introduction of oil-oxidizing microorganisms into contaminated soil: the problems and perspectives. Izvestiya Saratovskogo universiteta. Novaya seriya. Seriya: Khimiya. Biologiya. Ekologiya = Izvestiya of Saratov University. Chemistry. Biology. Ecology. 2011;11(2):102-111. (In Russian).
30. Korshunova T. Yu., Chetverikov S. P., Loginov O. N. Prospects of using a consortium of hydrocarbon oxidizing microorganisms for cleaning oil polluted soil of the extreme north. Teoreticheskaya i prikladnaya ekologiya = Theoretical and Applied Ecology. 2016;(1):88-94. (In Russian).
31. Domracheva L. I., Ashikhmina T. Ya., El'kina T. S., Gayfutdinova A. R. Microbial degradation of industrial waste (review). Teoreticheskaya i prikladnaya ekologiya = Theoretical and Applied Ecology. 2014; (2):6-16. (In Russian).
Review
For citations:
Fomicheva N.V., Smirnova Yu.D., Rabinovich G.Yu. Influence of new humic preparation on remediation of oil-contaminated soil. Proceedings of Universities. Applied Chemistry and Biotechnology. 2022;12(2):310-320. (In Russ.) https://doi.org/10.21285/2227-2925-2022-12-2-310-320
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