Study of macronutrients in hemp seeds during short-term germination

At present, hemp seeds are becoming increasingly popular as a source of nutrients. This work addressed the dynamics of macronutrients in the process of short-term germination of hempseeds by chemical and spectroscopic methods. Lyudmila 2021 cultivated hemp seeds along with hemp sprouts were used as objects of research. The germination of hemp seeds was carried out under laboratory conditions using special trays at 18–20 °C with the water added at a ratio of 2:1 for 5 days with periodic moistening. The obtained experimental data on the protein complex suggested that, in the studied interval of the germination of hemp seeds, the key hydrolytic decomposition of proteins occurs along with changes in structural components, including through the synthesis of new proteins accompanying the sprouting. The variations in such parameters as fat content, acid number and peak intensity of functional groups in the lipid fingerprint region (1745, 1157 and 1140 cm-1) indicated the accumulation of fatty acids as a result of the hydrolysis of triglycerides. The analysis of the IR spectra of hemp sprouts and the intensity of the bands of the corresponding functional groups in the carbohydrate region (1200–680 cm-1) suggested the intensive hydrolytic decomposition of polysaccharides. The variation in the content of extractive matter in the aqueous solutions of hemp sprouts indicated the accumulation and utilisation of water-soluble substances at the early stages of germination. The data on the predominance of water- and salt-soluble protein fractions indicated an increase in the biological value of hemp seeds during short-term germination.

1. Schultz C. J., Lim W. L., Khor S. F., Neumann K. A., Schulz J. M., Ansari O., et al. Consumer and health-related traits of seed from selected commercial and breeding lines of industrial hemp Cannabis sativa L. Journal of Agriculture and Food Research. 2020;2:100025. https://doi.org/10.1016/j.jafr.2020.100025.

2. Fike J. Industrial hemp: renewed opportunities for an ancient crop. Critical Reviews in Plant Sciences. 2016;35(5-6):406-424. https://doi.org/10.1080/07352689.2016.1257842.

3. Irakli M., Tsaliki E., Kalivas A., Kleisiaris F., Sarrou E., Cook C. M. Effect οf genotype and growing year on the nutritional, phytochemical, and antioxidant properties of industrial hemp (Cannabis sativa L.) seeds. Antioxidants. 2019;8:491. https://doi.org10.3390/antiox8100491.

4. Vonapartis E., Aubin M.-P., Seguin P., Mus- tafa A. F., Charron J.-B. Seed composition of ten industrial hemp cultivars approved for production in Canada. Journal of Food Composition and Analysis. 2015;39:8-12. https://doi.org10.1016/j.jfca.2014.11.004.

5. Lan Y., Zha F., Peckrul A., Hanson B., John- son B., Rao J., et al. Genotype x environmental effects on yielding ability and seed chemical composition of industrial hemp (Cannabis sativa L.) varieties grown in North Dakota, USA. Journal of the American Oil Chemists’ Society. 2019;96:1417-1425. https://doi.org/10.1002/aocs.12291.

6. Shelenga T. V., Grigor’ev S. V., Baturin V. S., Sarana Yu. V. Biochemical research of cannabis accessions collected in various regions of Russia. Agrarnaya Rossiya. 2011;(2):6-9. (In Russian).

7. Frassinetti S., Moccia E., Caltavuturo L., Gabriele M., Longo V., Bellani L., et al. Nutraceutical potential of hemp (Cannabis sativa L.) seeds and sprouts. Food Chemistry. 2018;262:56-66. https://doi.org10.1016/j.foodchem.2018.04.078.

8. Leonard W., Zhang P., Ying D., Fang Z. Hempseed in food industry: nutritional value, health benefits, and industrial applications. Comprehensive Reviews in Food Science and Food Safety. 2020;19(1):282308. https://doi.org/10.1111/1541-4337.12517.

9. Cerino P., Buonerba C., Cannazza G., D’Auria J., Ottoni E., Fulgione A., et al. A review of hemp as food and nutritional supplement. Cannabis and Cannabinoid Research. 2021;6(1):19-27. https://doi.org/10.1089/can.2020.0001.

10. Barčauskaitė K., Žydelis R., Ruzgas R., Bakšinskaitė A., Tilvikienė V. The seeds of industrial hemp (Cannabis sativa L.) a source of minerals and biologically active compounds. Journal of Natural Fibers. 2022. https://doi.org/10.1080/15440478.2022.2084486.

11. EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies) scientific opinion of the panel on dietetic products, nutrition and allergies on a request from European Commission related to labelling reference intake values for n-3 and n-6 polyunsaturated fatty acids. EFSA Journal. 2009;8(3):1461. https://doi.org/10.2903/j.efsa.2010.1461.

12. Vasilyev A. V., Sharanova N. E., Kulakova S. N. Nutrimetabolomics – the new stage of biochemistry of nutrition. The role of nutrilipidomic analysis. Voprosy pitaniya = Problems of Nutrition. 2014;83(1):4-11. (In Russian). https://doi.org/10.24411/0042-8833-201400001.

13. Farinon B., Molinari R., Costantini L., Merendino N. The seed of industrial hemp (Cannabis sativa L.): nutritional quality and potential functionality for human health and nutrition. Nutrients. 2020;29(12):1935. https://doi.org/10.3390/nu12071935.

14. Zelenina O. N., Serkov V. A. Fat acid composition of seed oil in new varieties and hybrids of Middle Russian hemp. Vestnik Rossiiskoi akademii sel’skokhozyaistvennykh nauk. 2011;2:77-79. (In Russian).

15. Gushchina V. A., Smirnov A. D., Sologub N. N., Sologub I. I. Fatty acid composition of hemp seed oil during its cultivation in the forest-steppe zone of the Middle Volga region. Agrarnyi nauchnyi zhurnal = The Agrarian Scientific Journal. 2022;(4):4-8. (In Russian). http://dx.doi.org/10.28983/asj.y2022i4pp4-8.

16. Walker C. G., Jebb S. A., Calder P. C., Phil D. Stearidonic acid as a supplemental source of ω-3 polyunsaturated fatty acids to enhance status for improved human health. Nutrition. 2013;29(2):363-369. https://doi.org/10.1016/j.nut.2012.06.003.

17. Sukhorada T. I., Proidak M. N., Semynin A. C., Gerasimova S. A., Shabelny M. M. New oil hemp variety Omegadar-1. Maslichnye kul’tury. Nauchno-tekhnicheskii byulleten’ Vserossiiskogo nauchno-issledovatel’skogo instituta maslichnykh kul’tur. 2009;(1):147-150. (In Russian).

18. Wang X.-S., Tang C.-H., Yang X.-Q., Gao W.-R. Characterization, amino acid composition and in vitro digestibility of hemp (Cannabis sativa L.) proteins. Food Chemistry. 2008;107:11-18. https://doi.org/10.1016/j.foodchem.2007.06.064.

19. House J. D., Neufeld J., Leson G. Evaluating the quality of protein from hemp seed (Cannabis sativa L.) products through the use of the protein digestibility-corrected amino acid score method. Journal of Agricultural and Food Chemistry. 2010;58:1180111807. https://doi.org/10.1021/jf102636b.

20. Malomo S. A., Aluko R. E. A comparative study of the structural and functional properties of isolated hemp seed (Cannabis sativa L.) albumin and globulin fractions. Food Hydrocolloids. 2015;43:743-752. https://doi.org/10.1016/j.foodhyd.2014.08.001.

21. Teh S. S., Bekhit A. E. D. A., Carne A., Birch J. Antioxidant and ACE-inhibitory activities of hemp (Cannabis sativa L.) protein hydrolysates produced by the proteases AFP, HT, pro-G, actinidin and zingibain. Food Chemistry. 2016;203:199-206. https://doi.org/10.1016/j.foodchem.2016.02.057.

22. Girgih A. T., Udenigwe C. C., Aluko R. E. Reverse-phase HPLC separation of hemp seed (Cannabis sativa L.) protein hydrolysate produced peptide fractions with enhanced antioxidant capacity. Plant Foods for Human Nutrition. 2013;68:39-46. https://doi.org/10.1007/s11130-013-0340-6.

23. Han C., Yang P. Studies on the molecular mechanisms of seed germination. Proteomics. 2015;15(10):1671-1679. https://doi.org/10.1002/pmc201400375.

24. Samofalova L. A., Simonenkova A. P., Safronova O. V. Study of structure formation in extracts from germinating oilseeds according to the functional properties of the lipid complex. Vestnik tekhnologicheskogo universiteta. 2017;20(4):120-122. (In Russian).

25. Kazennova N. K., Shneider D. V., Kazennov I. V. Changes in the chemical composition of grain products during sprouting. Khleboprodukty. 2013;(10):5557. (In Russian).

26. Vellneuve S., Power K. A., Guévremont E., Mondor M., Tsao R., Wanasundara J. P. D., et al. Effect of a shot-time germination process on the nutrient composition, microbial counts and bread-making potential of whole flaxseed. Journal of Food Processing and Preservation. 2014;39(6):1574-1586. http://doi.org/10.1111/jfpp.12385.

27. Dyakov A. B. The physiology and ecology of flax: monograph. Krasnodar: Vserossiiskii nauchno-issledovatel’skii institut maslichnykh kul’tur im. V. S. Pustovoita; 2006, 214 p. (In Russian).