THE EFFECT OF LIQUID END VOLATILE EXTRACTIVES OF OREGANO (ORIGANUM VULGARE L.) ON THE ENTOMOPATHOGENIC BACTERIA STRAIN B. THURIENGIENSIS 0371

The effect of liquid and volatile extractives and antimicrobial action of oregano (Origanum vulgare L.) on the entomopathogenic bacteria strain B. thuriengiensis var. thuringiensis 0371 was studied. The liquid spore culture of B. thuringiensis 0371 strain, the collection variety of oregano 100.1 and hybrid g4 containing 75.5% and 52.0% of carvacrol respectively, have been selected as the specimens with high carvacrol content having antimicrobial effect due to phenolic compounds (thymol, carvacrol) presence. The dynamics of strain spores keeping on the leaf surface was determined according to the method of A.G. Kolchevsky. Quantitative estimation of liquid and volatile extractives and antimicrobial action of substances was performed by E.M. Danini. The study demonstrated that the volatile fractions of oregano extractives possess the antimicrobial activity against the strain of B. thuringiensis 0371. For r4 and 100.1 varieties on flowering phases this activity rise to the maximum, so the amount of strain colonies was decreased in 3.0 and 2.5 times respectively. Extractive substances of oregano variety 100.1 have the greatest antimicrobial activity in the budding phase, but hybrid variety r4 has the same in flowering stage. It was noted that volatile extractives can change the morphology of the 0371 strain colonies and reduce their size 1.6 - 2.6 times in the stages of budding and flowering. Volatile extractives of oregano 100.1 variety was found to affect the keeping of B. thuringiensis 0371 spores at plant processing in natural conditions. The quantity of viable spores on the leaves is reduced by 96.3% on the third day after treatment.

Origanum vulgare L, Bacillus thuringiensis, Origanum vulgare L, Bacillus thuringiensis, liquid end volatile extractives antimicrobial action, carvacrol, strain

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