Оценка противогрибковой и антиоксидантной активности экстрактов, полученных из дождевого червя (Perionyx excavatus) с использованием различных растворителей

В данном исследовании были оценены противогрибковые и антиоксидантные активности экстрактов из порошка дождевого червя (Perionyx excavatus), приготовленных с использованием различных растворителей, включая 80%-й этанол, 50%-й этанол и дистиллированную воду. Эффективность экстракции варьировалась от 18,5 до 21,2%, в то время как содержание общего белка колебалось от 64,8 до 67,5%. Примечательно, что водный экстракт продемонстрировал наивысшие значения как по эффективности экстракции, так и по содержанию белка. Анализ методом тонкослойной хроматографии выявил присутствие аминокислот и пептидов с Rf-значениями от 0,42 до 0,65. Спектры инфракрасной спектроскопии с преобразованием Фурье показали характерные пики, связанные со структурой белков. Электрофорез в полиакриламидном геле с додецилсульфатом натрия указал на наличие белков преимущественно с молекулярной массой ниже 50 кДа в экстрактах, особенно в экстракте с 80%-м этанолом, который в основном содержал белки с молекулярной массой ниже 30 кДа. Противогрибковые тесты против Candida albicans показали наивысшую эффективность у экстракта с 80%-м этанолом с диаметром зоны ингибирования 13.3 мм и минимальной ингибирующей концентрацией 75 мг/мл. Кроме того, экстракты продемонстрировали активность в отношении улавливания радикалов DPPH и ABTS, причем экстракт с 80%-м этанолом показал наивысший антиоксидантный потенциал со значениями IC50 231,3 мкг/мл для DPPH и 208,9 мкг/мл для ABTS. В заключение необходимо отметить, что экстракты из порошка дождевого червя проявили значительную биологическую активность, что делает их перспективными кандидатами для применения в фармацевтике и косметологии.

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