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Proceedings of Universities. Applied Chemistry and Biotechnology

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Vol 11, No 4 (2021)
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CHEMICAL SCIENCES

508-516 426
Abstract

A fundamental problem in materials science consists in establishing a relationship between the chemical composition, structure, and properties of materials. This issue can be solved through the study of multicomponent systems and the directed synthesis of promising compounds. Of practical interest here are active dielectrics that are based on complex oxide compounds, specifically molybdates. Among complex molybdates and tungstates, ternary caged molybdates of the following structural types are of greatest importance: nasicon, perovskite, langbeinite, etc. Due to their widely varying elemental and quantitative compositions, such molybdates are convenient models for structural and chemical design, as well as the establishment of “composition–structure– properties” genetic relationships. Bismuth-containing complex molybdate systems exhibit the formation of phases having ferro-piezoelectric, ionic, and other properties. In this work, the Rb2MoO4–Bi2(MoO4)3–Zr(MoO4)2 ter nary salt system was studied for the first time using the method of intersecting sections in the subsolidus region (450–650 ℃). To this end, quasibinary sections were identified; triangulation was performed. Ternary molybdates Rb5BiZr(MoO4)6 and Rb2BiZr2(MoO4)6,5 were formed in the system using a ceramic technology. These compounds are isostructural to the previously obtained REE molybdates (M5LnZr(MoO4)6) but contain trivalent bismuth instead of rare earth elements. The structure of Rb5BiZr(MoO4)6 was adjusted via the Rietveld refinement technique using the TOPAS 4.2 software package. The ternary molybdate crystallizes in a trigonal system, with the following unit cell parameters of the R`3c space group: a = 10.7756(2) and c = 39.0464(7) Å. According to the studies of thermal properties exhibited by M5BiZr(MoO4)6, these ternary molybdates undergo the first-order phase transition in the temperature range of 450–600 ºC. The IR and Raman spectra of M5BiZr(MoO4)6 reveal the crystallization of ternary molybdates in the R`3c space group. The conducted comparative characterization of M2MoO4–Bi2(MoO4)3–Zr(MoO4)2 phase diagrams suggests that the phase equilibria of these systems depend on the nature of molybdates of monovalent elements.

517-522 380
Abstract

The copolymers of 2,3-Dichloropropene with vinyl chloride, methyl methacrylate, and styrene of different compositions were obtained via free-radical copolymerization. The copolymerization constants for the comonomers were found from the dependence of the copolymer composition on the initial mixture content. An increase in the content of 2,3-Dichloropropene in the initial mixture was found to decrease the yield and intrinsic viscosity of the copolymer for all systems. The reactivity of 2,3-Dichloropropene in copolymerization reactions was assessed according to the reciprocals of the copolymerization constants of vinyl chloride, methyl methacrylate, and styrene, which indicate the reactivity of the dichlorinated monomer when interacting with comonomer radicals. It was found that 2,3-dichloropropene is the most active in the reaction with a styrene radical. However, its reactivity with a methyl methacrylate radical decreases by a factor of 0.88 as compared to the styrene radical. The lowest reactivity of 2,3-Dichloropropene is observed when interacting with a vinyl chloride radical. The synthesized copolymers can be further modified by replacing chlorine atoms with functional groups.

PHYSICOCHEMICAL BIOLOGY

523-530 984
Abstract

Abstract. Recent years have seen the development of bacterial resistance to currently available antibiotics, which necessitates a search for new antimicrobial agents. Amomum muricarpum Elmer is a widely used medicinal plant species in the genus Amomum (family Zingiberaceae) that is commonly found in Laos, the Philippines, China, and Vietnam. The present article describes the chemical composition and antimicrobial activity of essential oils extracted from the leaves and rhizomes of A. muricarpum from North Vietnam. The hydrodistilled essential oil was analyzed using gas chromatography and gas chromatography-mass spectrometry, with the broth microdilution method designed to evaluate its antimicrobial efficacy. The absolute yield of essential oils amounted to 0.11% and 0.13% (v/w) for leaves and rhizomes, respectively, on a dry weight basis. It was found that the leaves and rhizomes of A. muricarpum produce oils abounding in monoterpenes. Of the total identified volatile components in the leaf oil (97.18%), three main constituents include α-pinene (40.45%), linalool (12.34%), and β-pinene (10.31%). In the rhizome oil, the main constituents include α-pinene (48.10%), β-pinene (20.32%), and linalool (7.56%) of the total identified volatile components (98.08%). An antimicrobial activity test indicates that essential oils from the leaves and rhizome of A. muricarpum inhibit the growth of Staphylococcus aureus ATCC 25923, with a minimum inhibitory concentration (MIC) of 200 µg/ml. In addition, the rhizome essential oil also exhibits antimicrobial activity against Bacillus cereus ATCC 14579, with a MIC value of 200 µg/ml. The results indicate the potential of essential oils extracted from A. muricarpum as a source of antimicrobial agents.

531-539 589
Abstract

Oligopyridine based copper(II) complexes are of interest to scientists as possible anticancer agents due to promising cytotoxic and DNA binding/cleaving properties. In this study, copper(II) complex [Cu(phendione)L2]·C2H5OH with 1,10-phenanthroline-5,6-dione (phendione) and 4,5-dichloro-isothiazole-3-carboxylic acid (HL) was synthesized and characterized by elemental analysis, IR-spectroscopy, X-ray powder diffraction and single-crystal X-ray diffraction. According to X-ray diffraction data, obtained compound is mononuclear complex with square pyramidal coordination environment of the central atom which is surrounded by two isothiazolate molecules and one phendione ligand. The X-ray diffraction data are confirmed by IR-spectroscopy data showing the presence of characteristic stretching vibration bands of the carbonyl and carboxyl groups of oligopyridine ligand and isothiazolate ions, respectively. Density functional theory (DFT) calculations for complex were carried out using the ADF software package to perform geometry optimization and frequency calculations that were in a good agreement with experimental IR spectrum. Cytotoxicity of complex and initial reagents was tested in vitro against HepG2 (human hepatocellular carcinoma) and MCF-7 (human breast adenocarcinoma) cell lines. The complex showed high dose-dependent cytotoxic activity with the IC50 values of 0.60±0.03 µM and 0.96±0.13 µM, respectively, which is higher than the activity of cisplatin against these cell lines. The activity of the complex is due to the presence of phendione ligand, which exhibits a similar cytotoxic activity.

540-548 375
Abstract

The present study aims to develop and test procedures for detecting the DNA of dangerous human papillomaviruses (HPV) types 6 and 16 in water samples. The conserved segments of HPV 6 L1 and HPV 16 L1 nucleic acid sequences were studied using bioinformatic methods with the help of the NCBI (National Center for Biotechnology Information) database and the BioEdit program. A total of 135 nucleic acid sequences of HPV6 L1 and 945 nucleic acid sequences of HPV16 L1 were examined. Five pairs of specific primers were developed for the identified conserved segments of nucleic acid sequences using specialized programs (PerlPrimer v.1.1.21, FastPCR 6.6, and Primer3Plus). In addition, several procedures for collecting samples from various water bodies located near Listvyanka settlement (Lake Baikal) were tested. The samples were subjected to comprehensive purification from insoluble particles and bacterial contamination to be tested for the presence of HPV DNA via PCR analysis using primers complementary to the nucleic acid sequences of HPV6 L1 and HPV16 L1. The conducted studies revealed HPV 6 and HPV 16 DNA in the water samples. Due to the use of the developed and tested procedures for collecting and examining samples from various water sources in the Baikal Natural Territory followed by a PCR analysis, it was possible to detect the presence of dangerous viruses. The proposed procedure of testing water samples for the presence of HPV can be useful in developing effective monitoring of water bodies and wastewater both in Baikal and other regions.

549-560 517
Abstract

The initiation of strawberries into in vitro culture is known to be complicated by the inhibition of organogenesis by phenolic oxidation products. An important role in this process is given to the selection of growth regulators that increase meristematic cell activity and shoot proliferation at the stage of organogenesis induction. The present study aims to obtain a viable apical meristem culture of garden strawberry and to study the effect of different antioxidants (reduced glutathione (RG); a new preparation, i.e., a mechanical composite (MC) on the basis of biogenic silicon and green tea catechins and plant growth regulators (6-benzylaminopurine; thidiazuron) on the initiation of axillary shoot formation in strawberry meristem culture. Terminal buds containing an apical meristem and two leaf primordia isolated from the stolons of two garden strawberry cultivars (Sunny Meadow and Festival Chamomile) were used as primary explants for the initiation of strawberries into in vitro culture. It was found for the first time that the MC exhibits higher antioxidant activity as compared to reduced glutathione, reduces darkening of initial explants, as well as enhancing regeneration up to 13.0% at p ≤ 0.05. Furthermore, the best effect on the formation of microshoots per explant is observed toward the end of material introduction into in vitro culture when combining the MC with growth regulators in the culture medium. Here, the effect of strawberry cultivar on explant regeneration and the number of microshoots per explant are insignificant. It is concluded that the procedure for using the MC as an effective antioxidant during material initiation into the culture can be applied to the large-scale in vitro propagation of garden strawberries. Moreover, the technology for obtaining the MC from plant waste is environmentally friendly, which is a significant advantage for its use in in vitro technologies.

561-569 338
Abstract

The paper describes a theoretical basis developed for estimating the parameters of a steady-state biotechnological process characterized by nonlinear microorganism growth kinetics. This study aimed to obtain a common methodological basis for estimating input parameters that determine actual technology implementation, taking into account all possible restrictions on the concentration of incoming substrate Sf (g/l) and dilution rate D (h-1 ). The theory development was based on a mathematical model describing one of the most common processes of lactic acid production. This mathematical model includes three mass balance equations (for biomass, substrate, and product), as well as an equation of microorganism growth kinetics. The study established relations for calculating the ultimate value of the dilution rate D ult at a given Sf , relations for the maximum and minimum values of Sf , as well as Sf and D providing the maximum productivity value QP, g/(l·h), where QP = PD (P – product concentration, g/l). These relations were designed to calculate the parameters of possible process implementation for two options at the same value of QP: two values of D calculated for a given Sf and two values of Sf calculated for a given D. A numerical experiment is described using the constants of the mathematical model confirmed by foreign studies. This numerical experiment is illustrated using an Sf-D dependence pattern determining an acceptable value range for Sf and D, with the separate calculation of parameters according to Sf sections. For each of these sections, calculation formulas are provided. It is concluded that the developed theoretical basis is sufficiently general in nature to be applied to biotechnological processes that involve other kinetic relations, as well as microorganism strains creating by-products and using raw materials that are employed to reproduce the substrate in the process of synthesis.

570-580 362
Abstract

Studies into mitochondrial сomplexomes in various organisms provide an insight into the native organization of proteins and metabolic pathways in the organelles of the subject under study. “Complexome” is a relatively recent concept describing the proteome of protein complexes, supercomplexes, and oligomeric proteins. Complexome analysis is performed using current electrophoretic and mass spectrometric techniques, in particular, by two-dimensional electrophoresis (2D BN/SDS-PAGE) in combination with mass spectrometry (MS). Unlike 2D IEF/SDS-PAGE, this method enables analysis of not only hydrophilic proteins of the mitochondrial matrix, but also membrane proteins and their associations, thus expanding the possibilities of studying the organelle proteome. In the present work, the complexome of etiolated pea shoots was studied for the first time using 2D BN/SDS-PAGE followed by MALDI-TOF MS. To this end, 145 protein spots excised from the gel were analyzed; 110 polypeptides were identified and assigned to different functional groups. A densitometric analysis revealed that the major protein group comprised the enzymes of the mitochondrial energy system (1), accounting for an average of 43% of the total polypeptide content. The remaining 57% was primarily distributed among the following functional categories: pyruvate dehydrogenase complex and citric acid cycle (2); amino acid metabolism (3); nucleic acid processing (4); protein folding (5); antioxidant protection (6); carrier proteins (7); other proteins (8); proteins having unknown functions (9). The obtained data indicate the complex organization of the pea proteome. In addition to the enzymes of the OXPHOS system, the proteins of other functional categories are found to form supramolecular structures. It is suggested that the presence of proteins from other cellular compartments may indicate the interaction of mitochondria with the enzymes or structures of corresponding organelles. In general, the obtained data on the pea complexome represent a kind of a mitochondrial “passport” that reflects the native state of the proteome of organelles corresponding to their physiological status.

581-589 409
Abstract

Given the spread of bacterial and viral diseases in young farm animals, the use of interferons and drugs to stimulate their biosynthesis has gained relevance. In a previous study, we examined the effect of a veterinary drug Trametin produced on the basis of Trametes pubescens (Shumach.: Fr.) Pilat. on the biosynthesis of interferons in the blood of mice. The present work is aimed at studying the biosynthesis dynamics of α- and γ-interferons when using Trametin and studying its prophylactic activity in calves. It is shown that a single oral administration of Trametin in doses ranging from 15 to 60 mg/kg causes a dose-dependent induction and production of γ-interferon in the blood of mice, whose maximum content reaches 1337.0±93.0 pg/mL at 48 h after administering a dose of 30 mg/kg. With a Trametin dose increase from 15 to 30 mg/kg, the level of α-interferon production rises to 1388.0±84.0 pg/mL at 48 h after administration. At a Cycloferon dose of 4.5 mg/kg, the production level of α-interferon and γ-interferon amounts to 1455.47±84.2 and 1447.0±90.0 pg/mL, respectively. The immunostimulatory properties of Trametin are confirmed by a scientific and economic experiment conducted using immunocompromised calves. In our studies, an immunological test of calf blood performed prior to and following the administration of Trimetin and Cycloferon constitutes criteria for the prophylactic activity of these drugs. The prophylactic efficacy of Trametin is confirmed by an increase in phagocytic activity by 10.5%, phagocytic index by 61.8%, and phagocytic number by 52.8%. After Trametin administration, the bactericidal activity of the serum increases by 60%. Cycloferon exhibits a similar immunostimulatory effect. Nonspecific prophylaxis using Trametin is shown to reduce the incidence of bacterial and viral respiratory diseases in young calves and generally improve their immunity.

590-602 445
Abstract

Plant extracts rich in polyphenols can be used in the food industry as natural preservatives, extending the shelf life of prepared and semi-finished foods without chemical preservatives. In this paper, we investigate the polyphenolic composition, antioxidant activity and antibacterial properties of herbal extracts as part of food systems. The research objects were knot grass (Polýgonum aviculáre), marjoram (Oríganum), bur beggar-ticks (Bídenstripartíta), thyme (Thymus), whortleberry leaves (Vaccínium ida vítis), calendula (Calendula), sage (Salvia), chamomile flowers (Matricāriachamomīlla), eucalyptus (Eucalýptus) and bearberry (Arctostáphylosúva-úrsi). We determined the total content of phenolic compounds and flavonoids; the antioxidant activity by DPPH and FRAP methods; variations in the bacterial сontamination of animal raw materials over total bacterial count (TBC), coliform bacteria, yeast/fungi, salmonella and staphylococcus. Extracts of sage (1138±57 mg GA/100 g and 537±25 mg C/100 g), eucalyptus (1073±49 mg GA/100 g and 412±20 mg C/100 g), chamomile flowers (1002±36 mg GA/100 g and 493±22 mg C/100 g) and marjoram (1015±42 mg GA/100 g and 458±21 mg C/100 g) contain the largest amount of biologically active substances (phenols and flavonoids, respectively). Sage, eucalyptus and chamomile extracts demonstrate the highest antioxidant activity among the studied samples. Most of the studied extracts exhibit little or no effect on the organoleptic properties of finished products. In addition, chamomile flower, sage and eucalyptus extracts suppress the growth of pathogenic microorganisms in foods under experimental conditions. The microflora growth is significantly reduced when treating animal raw materials with calendula flowers, marjoram and thyme extracts. Extracts of sage, chamomile flowers, calendula flowers, marjoram and thyme can be recommended as components of food raw materials.

603-616 391
Abstract

Low-molecular peptides of collagen origin are of crucial importance for the body metabolism. This work aims to substantiate the use of the hydrolysates of collagen-containing fish raw materials (scale) for protein sports nutrition. Sublimated low-molecular weight peptide water-soluble and dried water-insoluble protein-mineral supplements from the sardine scale were obtained by enzymatic thermal hydrolysis. The biopotential of scale and auxiliary food additives (apple pomace, flaxseed cake, pine nuts) was investigated using standard methods. The protein content and amino acid composition of the peptide supplement and the mineral composition of the protein-mineral supplement were analysed; their high biopotential according to the above criteria was shown. The peptide supplement contains all essential amino acids having a biological value of 59.9%. The protein-mineral supplement contains no tryptophan but a high amount of calcium (22.2 g/100 g) and phosphorus (12.0 g/100 g). The high content of functional food ingredients (protein, pectin, cellulose, polyphenols, minerals, vitamin C) in auxiliary vegetable raw materials was shown. The shape of a sports nutrition product (chocolate glazed protein bar) was substantiated. The basic recipe of a 60 g protein bar having the required protein content, a given structure and high consumer properties was established. Having a protein content of 23.5%, the bar provides a 24.1% calorific value, which corresponds to the "protein-rich food" for sports nutrition according to GOST 34006-2016. The biological protein value in the bar increased relative to the peptide supplement by 12% and amounted to 71.38%. The developed protein sports nutrition bar is dietary balanced by the content of amino acids (isoleucine, leucine, valine, methionine and cystine, phenylalanine and tyrosine, tryptophan, lysine, threonine, alanine, arginine, histidine, glycine, carnosine, taurine, ornithine, citrulline), fibre, polyphenols, calcium and phosphorus and is recommended for athletes and people having an active lifestyle.

617-626 332
Abstract

We examine the agglutinating ability of five compounds, namely, A1, A2, A3, A4 and BS1, isolated from activated sludge on selective media typical of a number of dominant microbial cultures that contribute to the formation of microbial aggregates. The morphological properties of the isolates and their lectin activity, as well as the physiological and biochemical properties of individual isolates were studied; microorganisms in their composition were identified. We assessed the capacity of the isolates under study to synthesize an exopolysaccharide matrix, as well as the sedimentation of activated sludge under the action of the native solution and culture liquid of the BS1 isolate. Based on their capacity to agglutinate, the BS1 and A2 isolates were selected for further research as producers of extracellular lectins and objects of agglutination, respectively. The biophysiochemical properties and molecular-genetic identification of the BS1 isolate allowed the degree of identity with r. Bacillus to be defined (96.19%); for the A2 isolate, 92.93% identity with p. Shigella and p. Escherichia was determined. To assess the capacity to synthesize a biofilm matrix, the BS1 and A2 isolates were cultivated on an agar nutrient solution using Congo Red dye. According to the obtained results, the isolates are capable of synthesizing an exopolysaccharide matrix, the main component of bacterial biofilms. The research results on the sedimentation of activated sludge induced by the native solution and culture liquid of BS1 showed the following. The sedimentation rate of activated sludge increased significantly at the beginning of the process upon adding a BS1 cell suspension, while the introduction of the native solution of BS1 intensified the process following 5 minutes of contact. The obtained experimental data suggest that the media containing extracellular bacterial lectins can be effectively used as a coagulant (flocculant) for the sedimentation of activated sludge.

627-641 782
Abstract

115 samples of honey of various botanical types, geographical origin and harvest year (2019– 2021) were analysed using attenuated total reflection infrared spectroscopy of impaired total reflection, refractometry and biochemical analysis. Initial honey samples in liquid and crystallised states were investigated. Crystalline D-glucopyranose (glucose), D-fructopyranose (fructose), their 40% solutions and invert sugar were used as auxiliary substances. Biochemical analysis was used to determine the glucose content in honey samples. Based on the obtained data, a relationship between the results of biochemical analysis and refractometry (refractive index, the content of invert sugars, humidity) was established. We deduced equations that allow the content of glucose and fructose in honey to be evaluated by the refractive index. Studying honey by IR spectroscopy showed that all investigated samples, regardless of the botanical and geographical origins, can be classified into three groups dominated by: I – glucose, II – fructose and III – mixed, with a close content of two monosaccharides. This allowed the bands characteristic of α- and β-pyranose forms of glucose and fructose to be identified, as well as the nature of their changes depending on the ratio of both monosaccharides in honey as a result of their crystallisation to be assessed. It is noted that the ratio of monosaccharides determines not only the stability of the liquid crystal structure and crystallisation rate in honey but also their optical, biochemical and nutritional properties, which are important for the preferred use of honey in medical and pharmacopoeial practice, dietetics and cosmetology.

642-650 435
Abstract

The limited use of plant proteins for food is explained by their low bioavailability and poor digestibility by enzymes of the gastrointestinal tract. Partially reproduced enzymatic processes of limited proteolysis that occur during seed germination are used to modify and improve the edibility characteristics of seed proteins. The present work discusses the possibility of reducing the duration of seed germination processes by optimising the conditions and parameters of limited proteolysis. To optimise manufacturing high-quality final product, enzymes (additional to the natural enzymes in the seed) and proteolysis conditions (in this case, temperature), as well as added substances (hydrolysis activators), were selected. The influence of cysteine on the formation of domain structures of proteins (enzymes and globulins) was evaluated. The proposed expressions can be used to determine those fragments of protein molecules that form stable domains and become unstructured when exposed to enzymes. Optimal conditions for limited proteolysis were identified based on the physical mechanism of action of papain-like proteolytic enzymes on pea legumin LegA (3KSC, CAA10722). It is shown that the decomposition of protein secondary structures takes 6–8 times longer, since the formed hydrogen bonds limit the access of enzymes to the corresponding amino-acid residues. It is also demonstrated that the decomposition of hydrogen bonds, e.g. by preliminary heat treatment of proteins, will broaden the prospects for limited proteolysis.

651-662 469
Abstract

The study aims to synthesize and examine the biological activity of mono- and binuclear platinum (II) and palladium (II) complexes containing terminal and bridging nitrite ligands against the test cultures of Bacillus subtilis B4647, Aspergillus brasiliensis (niger) F679, Pseudomonas aeruginosa B8243, and Escherichia coli. Through the interaction of mononuclear platinum (II) and palladium (II) complexes, dimeric complexes having nitrite ligands were synthesized. The composition and structure of these complexes were established using elemental analysis, conductometry, potentiometry, cryoscopy, infrared spectroscopy, X-ray diffraction analysis, and X-ray fluorescence analysis. A way to coordinate nitrite ligands with the central atom was established. Antimicrobial and antifungal properties were evaluated according to the capability of the synthesized complexes to inhibit the activity of bacteria and fungi via diffusion in agar and in vitro dilution. The minimum inhibitory and bactericidal concentrations of the complexes suppressing the visible growth of microorganisms and fungi, as well as exhibiting their bactericidal effect, ranged from 62.5–125 μmol/dm3. The obtained results revealed a high activity of the palladium (II) binuclear complex of the non-electrolytic type and the platinum (II) binuclear complex of the cationic type. Unlike mononuclear complexes, palladium and platinum binuclear complexes demonstrate higher antibacterial activity. Antibacterial effectiveness exhibited by the palladium complex of the non-electrolytic type against bacteria Bacillus subtilis and Escherichia coli, as well as fungi Aspergillus niger, is more pronounced. The only exception is the antimicrobial activity of the palladium complex against Pseudomonas aeruginosa, which is comparable to that of the binuclear platinum complex of the cationic type. By changing the structure of the complex, the composition and charge of the inner sphere, the number of coordination centers, as well as the nature and denticity of ligands, it is possible to achieve a higher toxic effect of the complexes against bacteria and fungi.

CHEMICAL TECHNOLOGY

663-672 330
Abstract

Inorganic deposits formed during operation and intermediate storage contain radionuclides, whose removal during the chemical decontamination of spent ion-exchange resins used in filters for special water purification at nuclear power plants has proved to be a challenge. In such deposits, radionuclides of the corrosion group (58.60Co, 54Mn, 51Cr) are typically located in the crystal lattice of poorly soluble iron oxides. The present work discusses the possibility of using mechanochemical activation in the decontamination of spent ion-exchange resins polluted with deposits of activated corrosion products from structural materials. Samples of natural and synthesised on the surface of the KU-2-8 cation exchanger in the presence of the 57Co label magnetites were used as model deposits. It was shown that an increase in the duration of mechanochemical activation leads to an increase in the dissolution rate of magnetite in model decontamination solutions based on еthylenediaminetetraacetic acid disodium salt (Trilon B) and nitric acid. It was shown that, when using Trilon B, magnetite dissolves more efficiently, which is explained by the interaction between the oxide surface and organic complexing agents. It can be assumed that solid-phase reactions occur during the mechanochemical activation of magnetite in the presence of dry reagents (Trilon B, oxalic, ascorbic and citric acids). Therefore, a poorly soluble shell formed on the oxide surface hinders the dissolution at a low magnetite/solution ratio. Unlike the reagent-free activation, for magnetite activated in the presence of oxalic acid, an increase in the solution/magnetite ratio promotes the dissolution of iron oxides. Using the example of a model cation exchanger, it was shown that the rate and efficiency of decontamination of spent ion-exchange resins polluted with deposits containing activated corrosion products increase significantly after mechanochemical activation in the presence of oxalic acid.

673-680 430
Abstract

Solvent extraction is a method for purifying oil extracts, which are used as oil-plasticisers for industrial rubber goods, from cancerogenic hydrocarbons. The efficiency of this method is mainly determined by the selectivity and solvent properties of a separating agent. However, when carrying out a comparative analysis of promising extraction methods, it is necessary to use real-time methods for studying the composition of the resulting products. The purification efficiency of oil extracts from polycyclic aromatic components, including carcinogenic hydrocarbons (benzo[a]pyrene, benzo[e]pyrene, benzo[a]anthracene, chrysene, benzo[b]fluoranthene, etc.), was evaluated depending upon the nature of selective solvents. The structure-group composition of the purified oil extracts and those at the second-stage of purification was determined by FTIR spectroscopy. It was found that the extraction efficiency of the studied solvents towards polycyclic aromatic hydrocarbons from oil extracts increases in the following order: dimethyl sulfoxide < N-methylpyrrolidone + 10 wt% ethylene glycol <N-methylpyrrolidone +50 wt% triethylene glycol. When using a solvent comprising N-methylpyrrolidone + 50 wt% triethylene glycol, the proportion of polyalkyl-substituted and condensed aromatic structures in the purified oil extract decreases by 16.8%; the oil extract yield increases by over 25 wt% in contrast to extraction with N-methylpyrrolidone + ethylene glycol mixture, which meets the requirements of the European Union for oil extract purification (Directive No. 2005/69/EC). Therefore, we recommend the solvent comprising N-methylpyrrolidone + 50 wt% triethylene glycol for purifying oil extracts from components having a technogenic impact on the environment and human health and IR spectroscopy for efficiency assessment of solvent extraction of oil extracts.



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